Abstract
Aim:
To isolate and identify the active constituents of Erigeron brevisapus (Vant.) Hand.-Mazz.
Methods:
The chemical constituents were isolated by silica gel column chromatography and two new compounds were obtained. Their structures were elucidated by IR, MS, 1HNMR, 13CNMR, DEPT and 2DNMR. The injury of BCMEC (bovine cerebral microvascular endothelial cell) was determined by lactic dehydrogenase (LDH), the ability of the drugs anti-oxidation and scavenging oxidation of free radical was measured by colorimetric method.
Results:
Two new compounds have been identified as 1-O-methyl-3, 5-O-dicaffeoyl quinic acid methyl ester (III) and 5-O-caffeoyl quinic acid butyl ester (IV).
Conclusion:
Compounds III and IV are new compounds. Compound III can protect BCMEC injury by LPC.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / isolation & purification
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Antioxidants / pharmacology
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Asteraceae / chemistry*
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Caffeic Acids / chemistry
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Caffeic Acids / isolation & purification*
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Caffeic Acids / pharmacology
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Cattle
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Cell Separation
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Endothelium, Vascular / drug effects*
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Endothelium, Vascular / metabolism
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L-Lactate Dehydrogenase / metabolism
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Microcirculation / cytology
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Molecular Structure
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Plants, Medicinal / chemistry*
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Protective Agents / isolation & purification
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Protective Agents / pharmacology
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Quinic Acid / analogs & derivatives
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Quinic Acid / chemistry
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Quinic Acid / isolation & purification*
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Quinic Acid / pharmacology
Substances
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1-O-methyl-3,5-O-dicaffeoyl quinic acid methyl ester
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5-O-caffeoyl quinic acid butyl ester
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Antioxidants
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Caffeic Acids
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Protective Agents
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Quinic Acid
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L-Lactate Dehydrogenase