Structure and function of archaeal box C/D sRNP core proteins

Mohamed Aittaleb; Rumana Rashid; Qiong Chen; John R Palmer; Charles J Daniels; Hong Li

doi:10.1038/nsb905

Structure and function of archaeal box C/D sRNP core proteins

Nat Struct Biol. 2003 Apr;10(4):256-63. doi: 10.1038/nsb905.

Authors

Mohamed Aittaleb¹, Rumana Rashid, Qiong Chen, John R Palmer, Charles J Daniels, Hong Li

Affiliation

¹ Department of Chemistry and Biochemistry, Institute of Molecular Biophysics, Florida State University, Tallahassee, Florida 32306, USA.

PMID: 12598892
DOI: 10.1038/nsb905

Abstract

Nop56p and Nop58p are two core proteins of the box C/D snoRNPs that interact concurrently with fibrillarin and snoRNAs to function in enzyme assembly and catalysis. Here we report the 2.9 A resolution co-crystal structure of an archaeal homolog of Nop56p/Nop58p, Nop5p, in complex with fibrillarin from Archaeoglobus fulgidus (AF) and the methyl donor S-adenosyl-L-methionine. The N-terminal domain of Nop5p forms a complementary surface to fibrillarin that serves to anchor the catalytic subunit and to stabilize cofactor binding. A coiled coil in Nop5p mediates dimerization of two fibrillarin-Nop5p heterodimers for optimal interactions with bipartite box C/D RNAs. Structural analysis and complementary biochemical data demonstrate that the conserved C-terminal domain of Nop5p harbors RNA-binding sites. A model of box C/D snoRNP assembly is proposed based on the presented structural and biochemical data.

MeSH terms

Amino Acid Sequence
Archaeal Proteins / chemistry*
Archaeal Proteins / genetics
Archaeal Proteins / metabolism*
Archaeoglobus fulgidus / genetics
Archaeoglobus fulgidus / metabolism
Binding Sites
Chromosomal Proteins, Non-Histone / chemistry
Chromosomal Proteins, Non-Histone / metabolism
Crystallography, X-Ray
Dimerization
Electrophoretic Mobility Shift Assay
Macromolecular Substances
Models, Molecular
Molecular Sequence Data
Molecular Structure
Nuclear Proteins*
Protein Structure, Tertiary
RNA Editing
RNA, Archaeal / chemistry*
RNA, Archaeal / genetics
RNA, Archaeal / metabolism*
RNA, Small Nucleolar / chemistry
RNA, Small Nucleolar / genetics
RNA, Small Nucleolar / metabolism
RNA, Small Untranslated
Ribonucleoproteins, Small Nucleolar / chemistry
Ribonucleoproteins, Small Nucleolar / genetics
Ribonucleoproteins, Small Nucleolar / metabolism*
Sequence Homology, Amino Acid
Static Electricity

Substances

Archaeal Proteins
Chromosomal Proteins, Non-Histone
Macromolecular Substances
Nuclear Proteins
RNA, Archaeal
RNA, Small Nucleolar
Ribonucleoproteins, Small Nucleolar
fibrillarin
RNA, Small Untranslated

Associated data

PDB/1NT2