Objective: To construct recombinant plasmids expressing L, M, S and pre-S1-S protein of HBsAg.
Methods: Amplifying segments of S, pre-S2-S, pre-S1-pre-S2-S genes of HBV by PCR and amplifying segment of pre-S1-S by overlap extension PCR; inserting the segments into Rc/CMV and pSG5UTPL/Flag plasmids respectively and exploring their expressions by Western-Blot hybridization, identifying the inserting segments by sequencing.
Results: The sequences of the inserted segments were the same as the genes of S, pre-S2-S, pre-S1-pre-S2-S and pre-S1-S and the results of Western-Blot hybridization were positive for the aimed proteins.
Conclusion: We have gained 8 recombinant plasmids expressing S, M, L and pre-S1-S proteins with high efficacy.