T cell activation induced by cross-linking CD3 and CD28 leads to silencing of Epstein-Barr virus/C3d receptor (CR2/CD21) gene and protein expression

Immunobiology. 2002 Dec;206(5):528-36. doi: 10.1078/0171-2985-00201.

Abstract

Complement receptor II (CR2) also known as CD21 is the receptor for C3d on immune complexes. In humans it serves as a receptor for the Epstein-Barr virus (EBV). CR2 is expressed on B cells and in low density in the T cell lineage. EBV can infect T cells and EBV-positive T lymphomas have been described. Although CR2 mRNA is readily detectable in T cells, the function of CR2 in human T lymphocytes remains elusive. Here we have analyzed the expression of CR2 in normal and activated T cells. PCR analyses and immunofluorescence/confocal microscopy of peripheral blood T cells and of activated T cells shows considerable reduction in CR2 mRNA and protein expression upon activation. The downregulation of CR2 expression may modulate life span or immunological reactivity of T cells and the susceptibility of cells to infection by lymphotropic viruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Lymphocytes / immunology
  • CD28 Antigens / immunology
  • CD28 Antigens / metabolism
  • CD3 Complex / immunology
  • CD3 Complex / metabolism
  • Cell Line
  • Cross-Linking Reagents / pharmacology
  • Down-Regulation
  • Flow Cytometry
  • Gene Expression Regulation
  • Humans
  • Interleukin-2 / immunology
  • Interleukin-2 / pharmacology
  • Lymphocyte Activation / immunology*
  • Microscopy, Confocal
  • Polymerase Chain Reaction
  • Proteins / analysis
  • RNA, Messenger / analysis
  • Receptors, Complement 3d / analysis*
  • Receptors, Complement 3d / immunology*
  • Receptors, Complement 3d / metabolism
  • T-Lymphocytes / immunology*

Substances

  • CD28 Antigens
  • CD3 Complex
  • Cross-Linking Reagents
  • Interleukin-2
  • Proteins
  • RNA, Messenger
  • Receptors, Complement 3d