Abstract
Activity-directed fractionation of a CH(2)Cl(2)-MeOH (1:1) extract of Leucophyllum ambiguum led to the isolation of two new lignans designated with the trivial names of 2'-methoxykobusin (1) and 2'-methoxy-4' '-hydroxydemethoxykobusin (2). In addition, the known compounds kobusin (3), 2',2' '-dimethoxysesamin (4), trans-cinnamic acid, apigenin, and apigetrin were obtained. The identification of the novel analogues 1 and 2 was accomplished by spectral methods. The structure of 1 was unequivocally confirmed by X-ray analysis. Compounds 1-4 interacted with bovine-brain calmodulin and inhibited the activation of the calmodulin-dependent enzyme cAMP phosphodiesterase.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Apigenin
-
Brain / enzymology
-
Calmodulin / antagonists & inhibitors*
-
Cattle
-
Cinnamates / chemistry
-
Cinnamates / isolation & purification
-
Electrophoresis, Polyacrylamide Gel
-
Flavonoids / chemistry
-
Flavonoids / isolation & purification
-
Glycoproteins / chemistry
-
Glycoproteins / isolation & purification*
-
Glycoproteins / pharmacology
-
Lignans / chemistry
-
Lignans / isolation & purification*
-
Lignans / pharmacology
-
Mexico
-
Molecular Structure
-
Plants, Medicinal / chemistry*
-
Scrophulariaceae / chemistry*
-
Stereoisomerism
Substances
-
Calmodulin
-
Cinnamates
-
Flavonoids
-
Glycoproteins
-
Lignans
-
cinnamic acid
-
apigetrin
-
Apigenin