Abstract
To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type ammonium transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of OsAMT2;1 in an ammonium-uptake-defective yeast mutant showed that this gene encodes functional ammonium transporters. OsAMT2;1 was constitutively expressed in both roots and shoots irrespective of the supply of inorganic nitrogen to the medium, whereas OsAMT3;1 expression was relatively weak. A database search with the amino acid sequence of OsAMT2;1 showed that there are 10 putative OsAMT genes in rice, i.e. three each for OsAMT1, OsAMT2 and OsAMT3, respectively, and one for OsAMT4.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Ammonium Chloride / pharmacology
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Carrier Proteins / genetics*
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Cation Transport Proteins*
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Gene Expression Regulation, Plant / drug effects
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Molecular Sequence Data
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Multigene Family / genetics
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Nitrates / pharmacology
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Oryza / drug effects
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Oryza / genetics*
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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Potassium Compounds / pharmacology
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RNA, Messenger / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
Substances
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Carrier Proteins
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Cation Transport Proteins
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Nitrates
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Plant Proteins
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Potassium Compounds
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RNA, Messenger
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ammonium transporters, plant
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Ammonium Chloride
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potassium nitrate
Associated data
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GENBANK/AB051864
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GENBANK/AB083581
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GENBANK/AB083582