Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE

Annemarie Ledeboer; Anne Wierinckx; John G J M Bol; Sarah Floris; Chantal Renardel de Lavalette; Helga E De Vries; Timo K van den Berg; Christine D Dijkstra; Fred J H Tilders; Anne-Marie van dam

doi:10.1016/s0165-5728(03)00031-6

Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE

J Neuroimmunol. 2003 Mar;136(1-2):94-103. doi: 10.1016/s0165-5728(03)00031-6.

Authors

Annemarie Ledeboer¹, Anne Wierinckx, John G J M Bol, Sarah Floris, Chantal Renardel de Lavalette, Helga E De Vries, Timo K van den Berg, Christine D Dijkstra, Fred J H Tilders, Anne-Marie van dam

Affiliation

¹ Department of Medical Pharmacology, Research Institute Neurosciences, VU University Medical Center, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands.

PMID: 12620647
DOI: 10.1016/s0165-5728(03)00031-6

Abstract

Adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mediate leukocyte infiltration into the CNS, in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). Because exogenous interleukin-10 (IL-10) inhibits ICAM-1 and VCAM-1 expression and clinical EAE, we hypothesize that endogenous IL-10 signaling may suppress expression of adhesion molecules. In a rat model of chronic relapsing EAE, expression levels of IL-10 and its receptor (IL-10R1), ICAM-1 and VCAM-1 mRNA in the spinal cord are markedly increased, whereas levels of IL-10 mRNA remain relatively low. The temporal pattern of mRNA and protein expression showed marked differences between spinal cord levels. During relapse, IL-10, IL-10R1, ICAM-1, VCAM-1 mRNA levels and neurological scores show positive correlations. We conclude that endogenous IL-10 is not a crucial factor inhibiting adhesion molecule expression in this model.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Adhesion Molecules / genetics
Cell Adhesion Molecules / immunology
Cell Adhesion Molecules / metabolism*
Chemotaxis, Leukocyte / immunology*
Chronic Disease
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental / immunology
Encephalomyelitis, Autoimmune, Experimental / metabolism*
Encephalomyelitis, Autoimmune, Experimental / pathology
Gene Expression Regulation / genetics
Gene Expression Regulation / immunology
Immunohistochemistry
Intercellular Adhesion Molecule-1 / genetics
Intercellular Adhesion Molecule-1 / immunology
Intercellular Adhesion Molecule-1 / metabolism
Interleukin-10 / genetics
Interleukin-10 / immunology
Interleukin-10 / metabolism*
Male
Multiple Sclerosis, Relapsing-Remitting / immunology
Multiple Sclerosis, Relapsing-Remitting / metabolism*
Multiple Sclerosis, Relapsing-Remitting / pathology
RNA, Messenger / immunology
RNA, Messenger / metabolism
Rats
Rats, Inbred Strains
Reaction Time / immunology
Receptors, Interleukin / genetics
Receptors, Interleukin / immunology
Receptors, Interleukin / metabolism*
Receptors, Interleukin-10
Spinal Cord / immunology
Spinal Cord / metabolism*
Spinal Cord / pathology
Time Factors
Vascular Cell Adhesion Molecule-1 / genetics
Vascular Cell Adhesion Molecule-1 / immunology
Vascular Cell Adhesion Molecule-1 / metabolism

Substances

Cell Adhesion Molecules
RNA, Messenger
Receptors, Interleukin
Receptors, Interleukin-10
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Interleukin-10