Abstract
In this study, we investigated whether hematopoietic stem cells (HSC) and progenitors present in human cord blood can express nerve growth factor (NGF)-specific receptors, TrkA and p75. Our results showed a marked expression of TrkA and NGF in cord blood CD34(+) cells. A gradient of TrkA and NGF expression exists and is highest in cord blood CD34(+) cells, reduced in cord blood mononuclear cells (MNC) and minimal in mononuclear cells isolated from adult peripheral blood. Our findings suggest that NGF may play a role in the differentiation of hematopoietic progenitors and indicate a different requirement for NGF by immune cells, depending on their state of maturity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Antigens, CD34 / immunology*
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Cell Differentiation / immunology*
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Fetal Blood / cytology*
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Fetal Blood / immunology
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Flow Cytometry
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Fluorescent Antibody Technique
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Hematopoietic Stem Cells / immunology
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Hematopoietic Stem Cells / metabolism*
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Humans
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Infant, Newborn
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Leukocytes, Mononuclear / immunology
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Leukocytes, Mononuclear / metabolism
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Middle Aged
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Nerve Growth Factor / genetics
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Nerve Growth Factor / metabolism*
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RNA, Messenger / metabolism
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Receptor, Nerve Growth Factor
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Receptor, trkA / genetics
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Receptor, trkA / metabolism*
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Receptors, Nerve Growth Factor / genetics
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Receptors, Nerve Growth Factor / metabolism
Substances
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Antigens, CD34
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RNA, Messenger
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Receptor, Nerve Growth Factor
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Receptors, Nerve Growth Factor
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Nerve Growth Factor
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Receptor, trkA