Objective: To study the expression of soluble human leukocyte antigen G (sHLA-G) in trophoblast cells of spontaneous abortion after in vitro fertilization and embryo transfer (IVF-ET) and choriocarcinoma, and explore the relationship between sHLA-G expression in trophoblast cells and the early embryo development.
Methods: 14 cases of trophoblast cells of spontaneous abortion after IVF-ET and 6 cases of choriocarcinoma tissue has been detected, and which were compared with the 10 cases of trophoblast cells of normal villus.
Results: (1) One case (1/14) has positive sHLA-G expression in IVF-ET group, and other cases in this group are negative or faint sHLA-G expression; ALL cases in choriocarcinoma group are strong positive sHLA-G expression; All cases in normal villous group are positive sHLA-G expression. (2) sHLA-G is expressed on the cytotrophoblast cells, especially on the extravillus cytotrophablast. The expression level of HLA-G is obviously decreased in the spontaneous abortion group compared with normal group. For single cells, the staining on the choriocarinoma cells are stronger than that of the normal villus cells.
Conclusion: High expression of sHLA-G in choriocarcinoma trophoblast cells and in trophoblast cells of normal villus and low expression in spontaneous abortion trophoblast cells indicating sHLA-G in trophoblast cells has some relationship with the early embryo development.