Abstract
Unlike the pp65 protein of human cytomegalovirus (CMV), which has an immunodominant peptide, pp65(495-503), recognized by human CD8(+) cells in the context of HLA A*0201, the fine peptide specificity for CMV IE1 has shown no such immunodominance. With the use of transgenic HLA A*0201/Kb and HHD II mice, a selected pool of IE1 peptides, including IE1(p256-264), IE1(p297-304), and IE1(p316-324), were shown to stimulate cytolytic T-lymphocyte lysis in the context of HLA A*0201. Based on an intracellular gamma interferon response, IE1(p297-304), a previously unrecognized CD8 epitope, triggered a prominent response to CMV IE1 in HLA A*0201 subjects.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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CD8-Positive T-Lymphocytes / immunology*
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Cytomegalovirus / genetics
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Cytomegalovirus / immunology*
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Cytomegalovirus Infections / immunology
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Granulocyte-Macrophage Colony-Stimulating Factor / administration & dosage
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Granulocyte-Macrophage Colony-Stimulating Factor / genetics
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HLA-A Antigens / genetics*
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HLA-A2 Antigen
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Humans
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Immediate-Early Proteins / genetics
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Immediate-Early Proteins / immunology*
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Immunodominant Epitopes / genetics
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In Vitro Techniques
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Interferon-gamma / biosynthesis
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Mice
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Mice, Transgenic
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Phosphoproteins / administration & dosage
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Phosphoproteins / genetics
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Recombinant Proteins
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T-Lymphocytes, Cytotoxic / immunology
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / genetics
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Viral Matrix Proteins / administration & dosage
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Viral Matrix Proteins / genetics
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Viral Proteins*
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Viral Vaccines / administration & dosage
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Viral Vaccines / genetics
Substances
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HLA-A Antigens
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HLA-A*02:01 antigen
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HLA-A2 Antigen
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IE1 protein, cytomegalovirus
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Immediate-Early Proteins
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Immunodominant Epitopes
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Peptide Fragments
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Phosphoproteins
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Recombinant Proteins
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Vaccines, DNA
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Viral Matrix Proteins
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Viral Proteins
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Viral Vaccines
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cytomegalovirus matrix protein 65kDa
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Interferon-gamma
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Granulocyte-Macrophage Colony-Stimulating Factor