Abstract
To find anticancer agents from higher plants, DNA strand-scission assay method was employed for bioassay-guided fractionation as well as for screening the crude extracts. During the screening, an ethyl acetate extracts of the heartwood of Caesalpinia sappan L. (Leguminosae) exhibited potent DNA strand-scission activity. Therefore, the ethyl acetate extracts of the dried heartwood of C. sappan was subjected to the bioassay-guided fractionation, which led to the isolation of a known compound, brazilin (1) as the active constituent. In addition, caesalpine J (2) was also isolated as an inactive constituent.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic* / chemistry
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Antineoplastic Agents, Phytogenic* / isolation & purification
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Antineoplastic Agents, Phytogenic* / pharmacology
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Benzopyrans* / chemistry
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Benzopyrans* / isolation & purification
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Benzopyrans* / pharmacology
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Bridged-Ring Compounds* / chemistry
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Bridged-Ring Compounds* / isolation & purification
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Bridged-Ring Compounds* / pharmacology
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Caesalpinia / chemistry*
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DNA Damage
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Diterpenes* / chemistry
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Diterpenes* / isolation & purification
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Diterpenes* / pharmacology
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Drug Screening Assays, Antitumor
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Molecular Structure
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Plant Extracts / chemistry
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Plasmids / drug effects*
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Spectrophotometry, Ultraviolet
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Wood
Substances
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Antineoplastic Agents, Phytogenic
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Benzopyrans
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Bridged-Ring Compounds
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Diterpenes
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Plant Extracts
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caesalpin J
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brazilin