A preliminary linkage map of the hard tick, Ixodes scapularis

Insect Mol Biol. 2003 Apr;12(2):201-10. doi: 10.1046/j.1365-2583.2003.00402.x.

Abstract

A linkage map of the Ixodes scapularis genome was constructed, based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F1 intercross progeny from a single, field-collected P1 female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of approximately 109 bp, the relationship of physical to genetic distance was found to be approximately 300 kb/cM in the I. scapularis genome.

MeSH terms

  • Animals
  • Chromosome Mapping / methods
  • Crosses, Genetic
  • Female
  • Gene Library
  • Genes, Insect / genetics*
  • Genetic Markers
  • Genome
  • Ixodes / genetics*
  • Male
  • Microsatellite Repeats
  • Polymorphism, Single-Stranded Conformational
  • Random Amplified Polymorphic DNA Technique
  • Sequence Tagged Sites

Substances

  • Genetic Markers