Abstract
One new pterocarpanoid, crotafuran E (1), and three known compounds were isolated from the bark of Crotalaria pallida. The structure of 1 was determined by spectral methods. Two pterocarpanoids, crotafurans A (2) and B (3), previously isolated from this plant, showed significant concentration-dependent inhibitory effects on the NO production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage-like cells, with IC(50) values of 23.0 +/- 1.0 and 19.0 +/- 0.2 microM, respectively. Compound 3 also inhibited the LPS/interferon-gamma (IFN-gamma)-stimulated NO production in N9 microglial cells with an IC(50) value of 9.4 +/- 0.9 microM. Moreover, compound 2 produced a concentration-dependent inhibition of the release of beta-glucuronidase and lysozyme from rat neutrophils in response to formyl-Met-Leu-Phe/cytochalasin B (fMLP/CB) with IC(50) values of 7.8 +/- 1.4 and 9.5 +/- 2.1 microM, respectively.
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification*
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology
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Crotalaria / chemistry*
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Cytochalasin B / pharmacology
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Furans / chemistry
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Furans / isolation & purification*
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Furans / pharmacology
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Heterocyclic Compounds, 4 or More Rings / chemistry
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Heterocyclic Compounds, 4 or More Rings / isolation & purification*
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Heterocyclic Compounds, 4 or More Rings / pharmacology
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Inhibitory Concentration 50
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Interferon-gamma / pharmacology
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Molecular Structure
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N-Formylmethionine Leucyl-Phenylalanine / pharmacology
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Neutrophils / drug effects
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Nitric Oxide / metabolism
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Nuclear Magnetic Resonance, Biomolecular
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Plant Bark / chemistry
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Plants, Medicinal / chemistry*
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Rats
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Taiwan
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Furans
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Heterocyclic Compounds, 4 or More Rings
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Lipopolysaccharides
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crotafuran E
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Nitric Oxide
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Cytochalasin B
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N-Formylmethionine Leucyl-Phenylalanine
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Interferon-gamma