Objective: Constructing a plasmid containing the shRNA of luciferase to suppress the expression of luciferase in BHK-21 cell.
Methods: A 334 bp human U6 snRNA promoter was amplified from human genomic DNA by PCR and ligated to a 21 bp reverse repeated motif of luciferase target sequence with 9 bp spacer and AAV plasmid pSNAV. The recombinant pSNAV/U6/Luc plasmid cotransfected with pMAMneoLuc or transfected luciferase cell line to detect the effect of luciferase expression separately.
Results: pSNAV/U6/luc suppresses the luciferase expression from pMAMneoLuc by 50% and luciferase cell line by 70%.
Conclusions: The results showed that the short hairpin RNA of luciferase can efficiently suppress its expression in BHK-21.