Abstract
Rubiscolin-6 (YPLDLF) is a delta selective opioid peptide isolated from the enzymatic digests of ribulose bisphosphate carboxylase/oxygenase (Rubisco) from spinach leaves. In a step-through type passive avoidance test in ddY mice, rubiscolin-6 enhanced memory consolidation at doses of 3nmol/mouse after intracerebroventricular administration, and at 100mg/kg after oral administration. These doses are smaller than the optimal doses for an analgesic effect. The memory enhancing effect of rubiscolin-6 was blocked by pretreatment with the delta antagonist naltrindole, suggesting the involvement of the delta opioid receptor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Oral
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Animals
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Avoidance Learning / drug effects
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Dose-Response Relationship, Drug
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Injections, Intraventricular
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Male
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Memory / drug effects*
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Memory / physiology
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Mice
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Naltrexone / analogs & derivatives*
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Naltrexone / pharmacology
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Narcotic Antagonists / pharmacology
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Peptide Fragments / pharmacology*
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Plant Proteins / chemistry
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Plant Proteins / pharmacology
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Receptors, Opioid, delta / agonists*
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Receptors, Opioid, delta / antagonists & inhibitors
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Ribulose-Bisphosphate Carboxylase / pharmacology*
Substances
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Narcotic Antagonists
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Peptide Fragments
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Plant Proteins
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Receptors, Opioid, delta
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rubiscolin 5
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rubiscolin 6
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Naltrexone
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Ribulose-Bisphosphate Carboxylase
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naltrindole