Structure and assembly of an augmented Sm-like archaeal protein 14-mer

Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4539-44. doi: 10.1073/pnas.0538042100. Epub 2003 Mar 31.

Abstract

To better understand the roles of Sm proteins in forming the cores of many RNA-processing ribonucleoproteins, we determined the crystal structure of an atypical Sm-like archaeal protein (SmAP3) in which the conserved Sm domain is augmented by a previously uncharacterized, mixed alpha/beta C-terminal domain. The structure reveals an unexpected SmAP3 14-mer that is perforated by a cylindrical pore and is bound to 14 cadmium (Cd(2+)) ions. Individual heptamers adopt either "apical" or "equatorial" conformations that chelate Cd(2+) differently. SmAP3 forms supraheptameric oligomers (SmAP3)(n = 7,14,28) in solution, and assembly of the asymmetric 14-mer is modulated by differential divalent cation-binding in apical and equatorial subunits. Phylogenetic and sequence analyses substantiate SmAP3s as a unique subset of SmAPs. These results distinguish SmAP3s from other Sm proteins and provide a model for the structure and properties of Sm proteins >100 residues in length, e.g., several human Sm proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Archaeal Proteins / chemistry*
  • Archaeal Proteins / genetics
  • Archaeal Proteins / metabolism
  • Binding Sites
  • Biophysical Phenomena
  • Biophysics
  • Cadmium / metabolism
  • Humans
  • Ligands
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Conformation
  • Protein Structure, Quaternary
  • Protein Structure, Tertiary
  • Protein Subunits
  • Sequence Homology, Amino Acid
  • Static Electricity
  • Thermoproteaceae / chemistry
  • Thermoproteaceae / genetics

Substances

  • Archaeal Proteins
  • Ligands
  • Protein Subunits
  • SmAP protein, Pyrobaculum aerophilum
  • Cadmium

Associated data

  • PDB/1M5Q