Inoculation with Actinomyces pyogenes and administration of prostaglandin (PG)F(2alpha) were used to induce late embryonic mortality (LEM) in heifers (n=8) on Days 30-38 of pregnancy in order to compare the profile for bovine pregnancy associated glycoprotein 1 (PAG1), progesterone and 15-keto-13,14-dihydro-PGF(2alpha) (PGFM). Two pregnant heifers were used as controls. Inoculation into the uterine body caused LEM, as established by ultrasonography in each heifer within 24h of treatment. When the inoculum was injected into the first part of the cervix, LEM occurred in one of two heifers (Heifer A) between 48 and 72 h after treatment. Similarly, PGF(2alpha) treatment caused LEM in three of four heifers. In six of eight heifers, PAG1 started to decrease steadily when it was accompanied by the subsequent death of the embryo. Inoculation through the cervix caused luteolysis in three of four heifers within 6-10 days after induction. After induction of LEM, PGFM concentrations showed a two to 3.8 fold increase in three of four heifers during the following six days, and from that time changed within normal ranges. The results of this study indicate that a PAG1 assay may provide an alternative method to ultrasonography for determining LEM in the cow.