A new structural motif in the left terminal domain of large viroids identified by covariation analysis

Virus Genes. 2003 Jan;26(1):19-23. doi: 10.1023/a:1022369803448.

Abstract

Sequence alignment of non-hammerhead viroids (Pospiviroidae) reveals that they can be divided into large and small viroids. Covariation analysis of these groups provides evidence for a rod-like secondary structure, but also for two proposed suboptimal structures, namely hairpin I and a bifurcation in the left terminus of large viroids. The strongest covariation within the branched T1 domain of all large Pospiviroidae indicates a new tertiary interaction, resulting in a double-pseudoknot within the (+) strand or an additional hairpin in the (-) strand. The structural element is expected to play a role in the viroid's life cycle.

Publication types

  • Comparative Study

MeSH terms

  • Algorithms
  • Base Sequence
  • Molecular Sequence Data
  • Nucleic Acid Conformation*
  • Phylogeny
  • RNA / chemistry*
  • RNA, Circular
  • RNA, Viral / chemistry*
  • Sequence Alignment
  • Sequence Analysis, RNA
  • Sequence Homology, Nucleic Acid
  • Viroids / chemistry*
  • Viroids / classification

Substances

  • RNA, Circular
  • RNA, Viral
  • RNA