Use of equivocal zone in interpretation of results of the amplified mycobacterium tuberculosis direct test for diagnosis of tuberculosis

A Kerleguer; J-L Koeck; M Fabre; P Gérôme; R Teyssou; V Hervé

doi:10.1128/JCM.41.4.1783-1784.2003

Use of equivocal zone in interpretation of results of the amplified mycobacterium tuberculosis direct test for diagnosis of tuberculosis

J Clin Microbiol. 2003 Apr;41(4):1783-4. doi: 10.1128/JCM.41.4.1783-1784.2003.

Authors

A Kerleguer¹, J-L Koeck, M Fabre, P Gérôme, R Teyssou, V Hervé

Affiliation

¹ HIA Val de GrAce, 75005 Paris. HIA Percy, 92140 Clamart. HIA Desgenettes, 69003 Lyon, France. [email protected]

Abstract

The sensitivity and specificity of the Amplified Mycobacterium Tuberculosis Direct (AMTD) test, evaluated with 1,363 respiratory samples (128 from tuberculous patients), were 92.97 and 98.7%, respectively. When an equivocal zone (30,000 to 1,000,000 relative light units [RLU]) was used instead of a 30,000-RLU cutoff, the sensitivity and specificity of the AMTD test were 92.97 and 100%, respectively.

Publication types

Evaluation Study

MeSH terms

DNA Probes
Humans
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / isolation & purification*
Nucleic Acid Amplification Techniques / methods*
RNA, Bacterial / isolation & purification
RNA, Ribosomal / genetics
Sensitivity and Specificity
Tuberculosis / diagnosis*
Tuberculosis / microbiology*

Substances

DNA Probes
RNA, Bacterial
RNA, Ribosomal