Developmental effects of prenatal cocaine exposure on 5-HT1A receptors in male and female rat offspring

Dev Neurosci. 2002;24(6):522-30. doi: 10.1159/000069363.

Abstract

Prenatal cocaine exposure results in behavioral abnormalities throughout development in rats, but little is known regarding the biological mechanisms underlying these abnormalities. Pregnant rats received subcutaneous twice-daily injections (1 ml/kg) of normal saline or 15 mg/kg of cocaine hydrochloride throughout gestation (gestation days 1-20). Following delivery, pups were placed with untreated surrogates. Male and female pups were killed on postnatal days 30, 60 or 120 for assessment of 5-HT(1A) receptor development in the forebrain, diencephalon, midbrain and pons using radiolabel immunocytochemistry. Findings revealed gender and age differences in developmental regulation of 5-HT(1A) receptors, indicating that male rats are more susceptible to long-term consequences of prenatal cocaine exposure in comparison to females. This study also demonstrates gender-specific development of serotonin (5-HT(1A)) receptors across postnatal ages, demonstrating a fundamentally different pattern of development of 5-HT(1A) receptors between males and females.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Age Factors
  • Animals
  • Brain / drug effects*
  • Brain / growth & development
  • Cocaine / toxicity*
  • Dopamine Uptake Inhibitors / toxicity*
  • Female
  • Immunohistochemistry
  • Male
  • Pregnancy
  • Prenatal Exposure Delayed Effects*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Serotonin / drug effects*
  • Receptors, Serotonin / metabolism
  • Receptors, Serotonin, 5-HT1
  • Sex Characteristics

Substances

  • Dopamine Uptake Inhibitors
  • Receptors, Serotonin
  • Receptors, Serotonin, 5-HT1
  • Cocaine