Cutting edge: conventional CD8 alpha+ dendritic cells are preferentially involved in CTL priming after footpad infection with herpes simplex virus-1

J Immunol. 2003 May 1;170(9):4437-40. doi: 10.4049/jimmunol.170.9.4437.

Abstract

CTL play a major role in immunity to HSV type 1, but little is known about the priming process. In this study, we have examined the class I-restricted presentation of an immunodominant determinant from HSV-1 glycoprotein B after footpad infection. We have found that the only cell types capable of presenting this determinant in draining popliteal lymph nodes within the first 3 days after infection are the CD11c(+)CD8alpha(+)CD45RA(-) dendritic cells. Given that such class I-restricted presentation is essential for CTL priming, this implies that these conventional CD8alpha(+) dendritic cells are the key subset involved in CTL immunity to this virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation / immunology
  • Antigen-Presenting Cells / immunology
  • Antigen-Presenting Cells / metabolism
  • Antigen-Presenting Cells / virology
  • CD8 Antigens / biosynthesis*
  • Cytotoxicity, Immunologic / immunology*
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Dendritic Cells / virology
  • Epitopes, T-Lymphocyte / immunology
  • Epitopes, T-Lymphocyte / metabolism
  • Herpes Simplex / immunology*
  • Herpes Simplex / virology
  • Herpesvirus 1, Human / immunology*
  • Hindlimb
  • Histocompatibility Antigens Class I / metabolism
  • Hybridomas
  • Immunity, Active
  • Immunodominant Epitopes / immunology
  • Immunodominant Epitopes / metabolism
  • Injections, Subcutaneous
  • Lymphocyte Activation / immunology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • T-Lymphocytes, Cytotoxic / immunology*
  • Viral Envelope Proteins / immunology
  • Viral Envelope Proteins / metabolism

Substances

  • CD8 Antigens
  • CD8alpha antigen
  • Epitopes, T-Lymphocyte
  • Histocompatibility Antigens Class I
  • Immunodominant Epitopes
  • Viral Envelope Proteins
  • glycoprotein B, Simplexvirus