Abstract
CTL play a major role in immunity to HSV type 1, but little is known about the priming process. In this study, we have examined the class I-restricted presentation of an immunodominant determinant from HSV-1 glycoprotein B after footpad infection. We have found that the only cell types capable of presenting this determinant in draining popliteal lymph nodes within the first 3 days after infection are the CD11c(+)CD8alpha(+)CD45RA(-) dendritic cells. Given that such class I-restricted presentation is essential for CTL priming, this implies that these conventional CD8alpha(+) dendritic cells are the key subset involved in CTL immunity to this virus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigen Presentation / immunology
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Antigen-Presenting Cells / immunology
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Antigen-Presenting Cells / metabolism
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Antigen-Presenting Cells / virology
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CD8 Antigens / biosynthesis*
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Cytotoxicity, Immunologic / immunology*
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Dendritic Cells / immunology*
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Dendritic Cells / metabolism*
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Dendritic Cells / virology
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Epitopes, T-Lymphocyte / immunology
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Epitopes, T-Lymphocyte / metabolism
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Herpes Simplex / immunology*
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Herpes Simplex / virology
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Herpesvirus 1, Human / immunology*
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Hindlimb
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Histocompatibility Antigens Class I / metabolism
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Hybridomas
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Immunity, Active
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Immunodominant Epitopes / immunology
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Immunodominant Epitopes / metabolism
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Injections, Subcutaneous
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Lymphocyte Activation / immunology
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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T-Lymphocytes, Cytotoxic / immunology*
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Viral Envelope Proteins / immunology
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Viral Envelope Proteins / metabolism
Substances
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CD8 Antigens
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CD8alpha antigen
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Epitopes, T-Lymphocyte
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Histocompatibility Antigens Class I
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Immunodominant Epitopes
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Viral Envelope Proteins
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glycoprotein B, Simplexvirus