The mammalian two-hybrid assay for detection of coactivator-nuclear receptor interactions

Methods Mol Med. 2003:85:175-83. doi: 10.1385/1-59259-380-1:175.
No abstract available

Publication types

  • Review

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Cell Line
  • Chloramphenicol O-Acetyltransferase / analysis
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chlorocebus aethiops
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Drug Evaluation, Preclinical / methods*
  • Fibroblasts
  • Genes, Reporter
  • Herpes Simplex Virus Protein Vmw65 / genetics
  • Herpes Simplex Virus Protein Vmw65 / metabolism
  • Ligands
  • Luciferases / analysis
  • Luciferases / genetics
  • Mammals
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Nuclear Receptor Interacting Protein 1
  • Protein Binding
  • Protein Conformation
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Structure-Activity Relationship
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Two-Hybrid System Techniques*

Substances

  • Adaptor Proteins, Signal Transducing
  • DNA, Complementary
  • DNA-Binding Proteins
  • GAL4 protein, S cerevisiae
  • Herpes Simplex Virus Protein Vmw65
  • Ligands
  • Nuclear Proteins
  • Nuclear Receptor Interacting Protein 1
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Luciferases
  • Chloramphenicol O-Acetyltransferase