Symmetrical reconversion: measuring cross-correlation rates with enhanced accuracy

J Magn Reson. 2003 Apr;161(2):258-64. doi: 10.1016/s1090-7807(02)00190-8.

Abstract

A new strategy has been developed to measure cross-correlation rates with much enhanced accuracy. The method relies on the use of four complementary experiments. Errors due to pulse miscalibration and to uncontrolled attenuation factors associated with relaxation are cancelled out. Problems due to violations of the secular approximation are greatly alleviated. The method has been applied to the measurement of N/NH (CSA/DD) cross-correlated relaxation rates in human ubiquitin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anisotropy
  • Carbon Isotopes
  • Humans
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Ubiquitin / chemistry*

Substances

  • Carbon Isotopes
  • Nitrogen Isotopes
  • Ubiquitin