The results of complete identification revealed the phenotypical similarity of V. cholerae strains isolated from surface water reservoirs im Grozny in 1995 and 2000. The strains under study proved to be atoxigenic, hemolysis-positive and phage resistant. The modified method of subculturing from peptone water 1 to peptone water 2 was more labor-consuming, but it was used in the bacteriological laboratory of the specialized antiepidemic brigade with the aim of more profound study of open reservoirs for the presence of V. cholerae with the positive result.