CD38 ligation plays a direct role in the induction of IL-1beta, IL-6, and IL-10 secretion in resting human monocytes

Roberto Lande; Francesca Urbani; Beatrice Di Carlo; Giuseppe Sconocchia; Silvia Deaglio; Ada Funaro; Fabio Malavasi; Clara M Ausiello

doi:10.1016/s0008-8749(03)00025-x

CD38 ligation plays a direct role in the induction of IL-1beta, IL-6, and IL-10 secretion in resting human monocytes

Cell Immunol. 2002 Nov;220(1):30-8. doi: 10.1016/s0008-8749(03)00025-x.

Authors

Roberto Lande¹, Francesca Urbani, Beatrice Di Carlo, Giuseppe Sconocchia, Silvia Deaglio, Ada Funaro, Fabio Malavasi, Clara M Ausiello

Affiliation

¹ Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanitá, 00161, Rome, Italy

PMID: 12718937
DOI: 10.1016/s0008-8749(03)00025-x

Abstract

CD38 signaling, either induced by ligation with specific agonistic monoclonal antibody (mAb) or after interaction with CD31, its cognate counter-receptor, is involved in release of IL-1beta, IL-6, and IL-10 cytokines in resting human monocytes. CD38 ligation by the F(ab')(2) IB4 mAb did not induce signals relevant for cytokine secretion and the block of the Fcgamma receptor I (FcgammaRI) by anti-CD64 or FcgammaRII by anti-CD32 mAb did not inhibit CD38-mediated IL-1beta release. Dimerization or multimerization of the CD38 molecule by: (i) cross-linking of the receptor ligated by F(ab')(2) or by (ii) increasing CD38 expression by treating monocytes with IFNgamma were able to restore the truncated CD38-mediated signals involved in cytokine secretion. These data indicate that CD38 receptor-mediated signals operate directly suggesting a Fcgamma receptorial surface molecule independent activation pathway. The key element for the receptor mediated signaling is represented by surface density of CD38 on resting monocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ADP-ribosyl Cyclase / chemistry
ADP-ribosyl Cyclase / immunology
ADP-ribosyl Cyclase / physiology*
ADP-ribosyl Cyclase 1
Antibodies, Monoclonal / immunology
Antibodies, Monoclonal / pharmacology
Antigens, CD / chemistry
Antigens, CD / immunology
Antigens, CD / physiology*
Biopolymers
Cell Line
Dimerization
Humans
Immunoglobulin Fab Fragments / immunology
Interferon-gamma / pharmacology
Interleukin-1 / metabolism*
Interleukin-10 / metabolism*
Interleukin-6 / metabolism*
Membrane Glycoproteins
Monocytes / metabolism*
Platelet Endothelial Cell Adhesion Molecule-1 / physiology
Receptors, IgG / antagonists & inhibitors
Receptors, IgG / immunology
Signal Transduction

Substances

Antibodies, Monoclonal
Antigens, CD
Biopolymers
Immunoglobulin Fab Fragments
Interleukin-1
Interleukin-6
Membrane Glycoproteins
Platelet Endothelial Cell Adhesion Molecule-1
Receptors, IgG
Interleukin-10
Interferon-gamma
ADP-ribosyl Cyclase
CD38 protein, human
ADP-ribosyl Cyclase 1