Abstract
We isolated a neuronal calcium sensor 1/frequenin-like gene, Mg-NCS-1, from Magnaporthe grisea and evaluated the phenotypes of null-mutants of the gene. The putative Mg-NCS-1 protein showed high similarity to the other NCS-1 proteins. The null-mutants had normal growth and pathogenicity similar to the parental strain, but their growth was suppressed in high concentrations of Ca2+ or acidic conditions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Calcium / chemistry
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Calcium / pharmacology
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Calcium Signaling
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Calcium-Binding Proteins / genetics*
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Fungal Proteins / genetics
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Hydrogen-Ion Concentration
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Magnaporthe / genetics*
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Magnaporthe / growth & development
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Magnaporthe / pathogenicity
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Molecular Sequence Data
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Mutation
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Nerve Tissue Proteins / genetics
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Neuronal Calcium-Sensor Proteins
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Neuropeptides / genetics*
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Phenotype
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transformation, Genetic
Substances
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Calcium-Binding Proteins
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Fungal Proteins
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Nerve Tissue Proteins
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Neuronal Calcium-Sensor Proteins
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Neuropeptides
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frequenin calcium sensor proteins
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Calcium