Abstract
Using the pyrrolidine-5,5-trans-lactam template, we have designed small, neutral, mechanism-based inhibitors of hepatitis C NS3/4A protease. Compound 2b, with a spiro-cyclobutyl P1 substituent and an isopropyl carbonyl substituent at the lactam nitrogen, has an IC(50) value in the replicon cell-based assay of 3 microM.
MeSH terms
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Drug Design
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Drug Stability
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Inhibitory Concentration 50
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Lactams / chemical synthesis
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Lactams / pharmacology*
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Protease Inhibitors / chemical synthesis*
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Spiro Compounds / chemical synthesis
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Spiro Compounds / pharmacology
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Structure-Activity Relationship
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Viral Nonstructural Proteins / antagonists & inhibitors*
Substances
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Lactams
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NS3 protein, hepatitis C virus
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Protease Inhibitors
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Spiro Compounds
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Viral Nonstructural Proteins