Abstract
In the course of detecting the interaction protein of RBBP10 by yeast two-hybridization, we isolated a novel cDNA that encodes a putative human protein with yrdC domain. It is named human yrdC protein. Because the cDNA contains an open reading fragment (ORF) without a 5' in- frame stop codon, 5' RACE and 3' RACE were proceeded to produce the full-length cDNA. An 1825 bp cDNA was isolated from human placenta, which encodes a putative protein of 279 amino acids. The protein contains a sua5-yciO-yrdC domain. Blast analysis against the human genome database of Genbank revealed that the gene contains five exons, and assigned the gene to human chromosome 1p34.2. A transcript about 2.5 kb is ubiquitously expressed in human tissues. The gene is highly conserved during evolution.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Cell Cycle Proteins*
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Chromosomes, Human, Pair 1 / genetics
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Cloning, Molecular
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DNA, Complementary / genetics
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GTP-Binding Proteins / chemistry*
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GTP-Binding Proteins / genetics*
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Gene Expression Profiling
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Humans
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Intracellular Signaling Peptides and Proteins*
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Molecular Sequence Data
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Neoplasm Proteins*
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Organ Specificity
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Protein Binding
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Proteins / chemistry*
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Proteins / genetics*
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Proteins / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA-Binding Proteins
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Sequence Homology, Amino Acid
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Two-Hybrid System Techniques
Substances
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Carrier Proteins
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Cell Cycle Proteins
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DNA, Complementary
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Intracellular Signaling Peptides and Proteins
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Neoplasm Proteins
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Nerve Tissue Proteins
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Proteins
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RBBP9 protein, human
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RNA, Messenger
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RNA-Binding Proteins
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GTP-Binding Proteins
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YRDC protein, human