Angiotensin II type 2 receptor deficiency exacerbates heart failure and reduces survival after acute myocardial infarction in mice

Circulation. 2003 May 20;107(19):2406-8. doi: 10.1161/01.CIR.0000072763.98069.B4. Epub 2003 May 5.

Abstract

Background: Angiotensin II plays a prominent role in the progression of heart failure after acute myocardial infarction (AMI). Although both angiotensin type 1 (AT1) and type 2 (AT2) receptors are known to be present in the heart, comparatively little is known about the latter. We therefore examined the role played by AT2 receptors in post-AMI heart failure.

Methods and results: In wild-type mice subjected to AMI by coronary artery ligation, AT2 receptor immunoreactivity is upregulated in the infarct and border areas. Among AT2 receptor-null (-/-) mice, the 7-day survival rate after AMI was significantly lower than among wild-type mice (43% versus 67%; P<0.05). All sham-operated animals of both genotypes survived through the study. Ventricular mRNA levels for brain natriuretic peptide were elevated in both genotypes 24 hours after coronary occlusion, with levels in AT2-/- significantly higher than in wild-type mice, as were their lung weights, and histological examination revealed marked pulmonary congestion in the AT2-/- mice. Cardiac function was significantly decreased in AT2-/- mice 2 days after AMI.

Conclusions: AT2 receptor deficiency exacerbates short-term death rates and heart failure after experimental AMI in mice. The AT2 receptor may thus exert a protective effect on the heart after AMI.

MeSH terms

  • Acute Disease
  • Animals
  • Disease Models, Animal
  • Disease Progression
  • Echocardiography
  • Electrocardiography
  • Female
  • Heart Failure / diagnosis
  • Heart Failure / etiology
  • Heart Failure / physiopathology*
  • Homozygote
  • Ligation
  • Mice
  • Mice, Knockout
  • Myocardial Infarction / complications
  • Myocardial Infarction / diagnosis
  • Myocardial Infarction / physiopathology
  • Natriuretic Peptide, Brain / genetics
  • Natriuretic Peptide, Brain / metabolism
  • RNA, Messenger / metabolism
  • Receptor, Angiotensin, Type 1
  • Receptor, Angiotensin, Type 2
  • Receptors, Angiotensin / analysis
  • Receptors, Angiotensin / deficiency*
  • Receptors, Angiotensin / genetics
  • Receptors, Angiotensin / metabolism
  • Severity of Illness Index
  • Survival Rate

Substances

  • RNA, Messenger
  • Receptor, Angiotensin, Type 1
  • Receptor, Angiotensin, Type 2
  • Receptors, Angiotensin
  • Natriuretic Peptide, Brain