Abstract
Real-time PCR with melting curve analysis of PCR products is a rapid procedure for detecting and genotyping herpes simplex virus (HSV). When testing mucocutaneous samples for HSV by a real-time PCR assay targeting the DNA polymerase gene, we found that some PCR products had atypical melting curves that did not conform to the expected melting temperatures for HSV type 1 or 2. Sequence analysis showed that these strains had base-pair mismatches over the probe binding sites. An alternative assay is required to type such atypical isolates.
MeSH terms
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Base Pair Mismatch
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Base Sequence
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Binding Sites / genetics
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DNA Probes / genetics
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DNA-Directed DNA Polymerase / genetics
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Genes, Viral
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Genetic Variation
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Genotype
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Herpesvirus 1, Human / classification
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Herpesvirus 1, Human / enzymology
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Herpesvirus 1, Human / genetics
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Herpesvirus 1, Human / isolation & purification
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Herpesvirus 2, Human / classification
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Herpesvirus 2, Human / enzymology
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Herpesvirus 2, Human / genetics
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Herpesvirus 2, Human / isolation & purification
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Humans
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Molecular Sequence Data
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Nucleic Acid Denaturation
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Polymerase Chain Reaction / methods
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Sequence Homology, Nucleic Acid
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Simplexvirus / classification
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Simplexvirus / enzymology
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Simplexvirus / genetics*
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Simplexvirus / isolation & purification
Substances
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DNA Probes
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DNA-Directed DNA Polymerase