Abstract
LFA-1 (CD11a/CD18) plays a crucial role in various inflammatory responses. In this study, we show that LFA-1(-/-) mice are far more resistant to Listeria monocytogenes infection than LFA-1(+/-) mice. Consistent with this, we found the following: 1) the numbers of granulocytes infiltrating the liver were markedly higher in LFA-1(-/-) mice than in LFA-1(+/-) mice, 2) increased antilisterial resistance in LFA-1(-/-) mice was abrogated by depletion of granulocytes, and 3) the numbers of granulocytes in peripheral blood, and the serum levels of both G-CSF and IL-17 were higher in LFA-1(-/-) mice than in LFA-1(+/-) mice. Neither spontaneous apoptosis nor survival of granulocytes from LFA-1(-/-) mice were affected by physiological concentrations of G-CSF. Our data suggest regulatory effects of LFA-1 on G-CSF and IL-17 secretion, and as a corollary on neutrophilia. Consequently, we conclude that increased resistance of LFA-1(-/-) mice to listeriosis is due to neutrophilia facilitating liver infiltration by granulocytes promptly after L. monocytogenes infection, although it is LFA-1 independent.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Caspase 3
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Caspases / metabolism
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Cell Adhesion / genetics
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Cell Adhesion / immunology
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Granulocyte Colony-Stimulating Factor / biosynthesis
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Granulocyte Colony-Stimulating Factor / blood
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Granulocyte Colony-Stimulating Factor / physiology*
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Granulocytes / enzymology
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Granulocytes / immunology
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Granulocytes / pathology
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Immunity, Innate / genetics
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Immunosuppression Therapy
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Interleukin-17 / biosynthesis
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Interleukin-17 / blood
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Interleukin-17 / physiology*
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Leukocyte Count
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Leukocytosis / genetics*
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Leukocytosis / immunology*
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Leukocytosis / microbiology
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Leukocytosis / pathology
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Listeria / growth & development
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Listeria / immunology
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Listeriosis / genetics*
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Listeriosis / immunology*
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Listeriosis / microbiology
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Listeriosis / pathology
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Liver / immunology
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Liver / microbiology
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Liver / pathology
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Lung / blood supply
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Lung / pathology
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Lymphocyte Function-Associated Antigen-1 / genetics*
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Lymphocyte Function-Associated Antigen-1 / physiology
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Neutrophil Infiltration / genetics
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Neutrophil Infiltration / immunology
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Neutrophils / immunology*
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Neutrophils / pathology
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Phenotype
Substances
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Interleukin-17
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Lymphocyte Function-Associated Antigen-1
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Granulocyte Colony-Stimulating Factor
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Casp3 protein, mouse
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Caspase 3
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Caspases