Abstract
Overproduction of nitric oxide by inducible nitric oxide synthase (iNOS) acts cytotoxically and contributes to inflammation. We explored the roles of iNOS in the pathogenesis of Helicobacter pylori-associated diseases. Using reverse-transcribed PCR, we examined topographical patterns of iNOS mRNA expression in the gastroduodenal mucosa in H. pylori-negative controls and H. pylori-positive patients with duodenal ulcer (DU), gastric ulcer (GU), and ulcer-free gastritis. iNOS expression showed topographical variations among the tested disorders. As compared to controls, DU had a significantly higher expression of iNOS mRNA in the duodenum, GU in the antrum and duodenum, and gastritis in the antrum and corpus. H. pylori eradication yielded a significant reduction of iNOS mRNA in the duodenum of DU and in the antrum of GU. Diverse topographical patterns of H. pylori-induced iNOS expression may contribute to mechanisms by which H. pylori elicits different clinical disorders.
Publication types
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Clinical Trial
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Randomized Controlled Trial
MeSH terms
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Amoxicillin / administration & dosage
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Anti-Ulcer Agents / administration & dosage
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Biopsy
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Clarithromycin / administration & dosage
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Drug Therapy, Combination
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Duodenal Ulcer / drug therapy
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Duodenal Ulcer / enzymology
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Duodenal Ulcer / microbiology*
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Endoscopy, Digestive System
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Enzyme Induction / drug effects
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Enzyme Induction / physiology
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Gastric Mucosa / enzymology
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Gastric Mucosa / microbiology
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Gastric Mucosa / pathology
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Gastritis / drug therapy
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Gastritis / enzymology
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Gastritis / microbiology*
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Enzymologic / physiology
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Helicobacter pylori / drug effects
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Helicobacter pylori / physiology*
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Humans
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Immunoenzyme Techniques
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Intestinal Mucosa / enzymology
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Intestinal Mucosa / microbiology
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Intestinal Mucosa / pathology
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Nitric Oxide Synthase / biosynthesis*
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Nitric Oxide Synthase / genetics
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Nitric Oxide Synthase Type II
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Omeprazole / administration & dosage
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Pyloric Antrum / enzymology
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Pyloric Antrum / microbiology
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Pyloric Antrum / pathology
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RNA, Messenger / genetics
Substances
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Anti-Ulcer Agents
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RNA, Messenger
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Amoxicillin
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NOS2 protein, human
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Clarithromycin
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Omeprazole