A new dark quencher for use in genetic analysis

Jonathan P May; Lynda J Brown; Ivo Rudloff; Tom Brown

doi:10.1039/b300934c

A new dark quencher for use in genetic analysis

Chem Commun (Camb). 2003 Apr 21:(8):970-1. doi: 10.1039/b300934c.

Authors

Jonathan P May¹, Lynda J Brown, Ivo Rudloff, Tom Brown

Affiliation

¹ Department of Chemistry, University of Southampton, Highfield, Southampton, UK SO17 1BJ.

PMID: 12744324
DOI: 10.1039/b300934c

Abstract

A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anthraquinones / chemistry*
Carbocyanines / chemistry
Fluorescence Polarization
Genetic Techniques*
Oligonucleotide Probes / chemistry*
Oligonucleotides / chemistry
Spectrometry, Fluorescence / methods

Substances

Anthraquinones
Carbocyanines
Oligonucleotide Probes
Oligonucleotides
cyanine dye 5
1,4-diaminoanthraquinone