Effect of lysed Enterococcus faecalis FK-23 (LFK) on allergen-induced peritoneal accumulation of eosinophils in mice

Clin Exp Allergy. 2003 May;33(5):684-7. doi: 10.1046/j.1365-2222.2003.01654.x.

Abstract

Background: The interest in anti-allergy immunoregulation by lactic acid bacteria has been growing for the last few decades. There is some evidence to suggest that lysed Enterococcus faecalis FK-23 (LFK) could relieve the clinical symptoms of pollinosis. However, the mechanism responsible for this phenomenon remains unknown.

Objective: To identify the effect of LFK, a lysozyme treated and heat-killed preparation from the lactic acid bacteria Enterococcus faecalis FK-23 strain, on allergen-induced eosinophil accumulation.

Methods: BALB/c mice were sensitized with ragweed pollen extract, and peritoneal accumulation of eosinophils was induced. A total of 60 mg (0.5 mL) LFK was orally administered to the experimental mouse every day during 21 days of the sensitization period. In addition, LFK 4 mg, 25 mg and 60 mg (each 0.5 mL) were also orally administered to a mouse of each group every day for 21 days. Saline was fed in a dose of 0.5 mL/mouse per day for the same duration as a control.

Results: Compared with control mice, LFK-treated mice exhibited decreased ragweed pollen allergen-induced peritoneal accumulation of eosinophils (P = 0.013), which showed a tendency to be in a dose-dependent fashion (P = 0.14).

Conclusion: The results provide laboratory evidence of the role for LFK, a lactic acid bacteria preparation, in combating eosinophil accumulation.

MeSH terms

  • Administration, Oral
  • Allergens / immunology*
  • Ambrosia / immunology
  • Animals
  • Anti-Allergic Agents / therapeutic use*
  • Cell Movement
  • Dose-Response Relationship, Immunologic
  • Enterococcus faecalis / immunology*
  • Eosinophilia / etiology
  • Eosinophilia / immunology
  • Eosinophilia / prevention & control*
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Peritoneal Cavity / pathology
  • Pollen / immunology
  • Vaccines, Inactivated*

Substances

  • Allergens
  • Anti-Allergic Agents
  • Vaccines, Inactivated