Abstract
The development of T cells is thought to be independent of B cells. However, defects in cell-mediated immunity in individuals with B-cell deficiency suggest the contrary. To test whether B cells affect T-lymphocyte development, we constructed mice with a monoclonal T-cell compartment (MT) and monoclonal B- and T-cell compartments (MBTs). In these mice, the T cells expressed a DO 11.10 transgenic (DO-T) cell receptor restricted to major histocompatibility complex (MHC) class IId. While CD4+ DO-T lymphocytes are rare in transgenic H-2b MT mice, we found that in H-2b MBT mice under the influence of B cells, DO-T lymphocytes mature into large numbers of CD4+ peripheral T cells. H-2b MBT mice have more CD4+ thymocytes than H-2b MT mice. These data are consistent with the view that B cells play some role in thymocyte development.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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B-Lymphocyte Subsets / immunology
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B-Lymphocytes / immunology*
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CD4 Lymphocyte Count
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Clonal Deletion / immunology
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H-2 Antigens / immunology
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Histocompatibility Antigens Class II / genetics
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Histocompatibility Antigens Class II / immunology
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Immunity, Cellular
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Immunoglobulin lambda-Chains / genetics
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Immunoglobulin lambda-Chains / immunology
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Immunologic Deficiency Syndromes / genetics
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Immunologic Deficiency Syndromes / pathology
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Immunophenotyping
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Mice
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Mice, Knockout
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Mice, Transgenic
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Models, Immunological
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Ovalbumin / immunology
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Spleen / cytology
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Spleen / immunology
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology*
Substances
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H-2 Antigens
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Histocompatibility Antigens Class II
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I-Ad antigen
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Immunoglobulin lambda-Chains
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Ovalbumin