Abstract
We isolated a learning associated protein of slug with a molecular mass of 18 kDa (LAPS18) homologue from the expressed sequence tag database of Aplysia kurodai and named it Aplysia LAPS18-like protein (ApLLP). ApLLP encodes 120 amino acids and has 57% identity with LAPS18. To examine the subcellular expression pattern of ApLLP we constructed an EGFP-tagged ApLLP fusion protein and overexpressed it in both Aplysia neurons and COS-7 cells. In contrast to the previous findings, which showed that LAPS18 is secreted by COS-7 cells, ApLLP-EGFP was localized to the nucleus, and most of it to nucleoli. Analysis of deletion mutants of ApLLP-EGFP showed that the N-terminal and the C-terminal nucleolar and nucleus localization signal sequences are important for localization to the nucleus and the nucleoli.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aplysia / genetics*
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Base Sequence
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Behavior, Animal*
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COS Cells
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DNA, Complementary
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Gene Expression
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Green Fluorescent Proteins
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Learning / physiology*
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Luminescent Proteins
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Neurons / metabolism
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Nuclear Localization Signals / chemistry
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Nuclear Localization Signals / genetics*
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Polymerase Chain Reaction / methods
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Proteins / physiology*
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Recombinant Fusion Proteins / biosynthesis
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Sequence Alignment
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Sequence Deletion
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Sequence Homology, Amino Acid
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Software
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Subcellular Fractions / metabolism
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Transfection
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Viral Proteins / chemistry
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Viral Proteins / genetics
Substances
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DNA, Complementary
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LAPS18 protein, Limax marginatus
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Luminescent Proteins
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Nuclear Localization Signals
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Proteins
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Recombinant Fusion Proteins
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Viral Proteins
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Green Fluorescent Proteins