Cutting edge: Monarch-1: a pyrin/nucleotide-binding domain/leucine-rich repeat protein that controls classical and nonclassical MHC class I genes

J Immunol. 2003 Jun 1;170(11):5354-8. doi: 10.4049/jimmunol.170.11.5354.

Abstract

Proteins containing a limited number of N-terminal motifs followed by nucleotide-binding domain and leucine-rich repeat regions are emerging as important regulators for immunity. A search of human genome scaffold databases has identified a large family of known and unknown genes, which we have recently called the CATERPILLER (caspase recruitment domain, transcription enhancer, r(purine)-binding, pyrin, lots of leucine repeats) gene family. This work describes the characterization of a new member, Monarch-1. Monarch-1 has four different splice forms due to the differential splicing of leucine-rich repeat motifs. It is expressed in cells of myeloid-monocytic origin. Affymetrix microarrays and small interfering RNA were used to elucidate the downstream effects of Monarch-1 expression in cells including those of myeloid-monocytic origin. These analyses show that Monarch-1 enhances nonclassical and classical MHC class I expression at the level of the promoter, RNA, and protein expression.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Carrier Proteins / biosynthesis
  • Carrier Proteins / chemistry*
  • Carrier Proteins / genetics
  • Carrier Proteins / physiology
  • Cloning, Molecular
  • Cytoskeletal Proteins
  • DNA, Complementary / isolation & purification
  • Gene Expression Profiling
  • Genes, MHC Class I*
  • HeLa Cells
  • Humans
  • Intracellular Signaling Peptides and Proteins*
  • Leucine*
  • Leucine-Rich Repeat Proteins
  • Molecular Sequence Data
  • Monocytes / immunology
  • Monocytes / metabolism
  • Myeloid Cells / immunology
  • Myeloid Cells / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Protein Binding
  • Protein Biosynthesis
  • Protein Structure, Tertiary / genetics
  • Proteins / chemistry*
  • Proteins / genetics
  • Proteins / physiology*
  • Purine Nucleotides / chemistry*
  • Pyrin
  • Repetitive Sequences, Amino Acid*
  • Reverse Transcriptase Polymerase Chain Reaction
  • U937 Cells

Substances

  • Carrier Proteins
  • Cytoskeletal Proteins
  • DNA, Complementary
  • Intracellular Signaling Peptides and Proteins
  • Leucine-Rich Repeat Proteins
  • MEFV protein, human
  • NLRP12 protein, human
  • Proteins
  • Purine Nucleotides
  • Pyrin
  • Leucine

Associated data

  • GENBANK/AY116204
  • GENBANK/AY116205
  • GENBANK/AY116206
  • GENBANK/AY116207