Abstract
Alternative splicing has recently emerged as a major mechanism of generating protein diversity in higher eukaryotes. We compared alternative splicing isoforms of 166 pairs of orthologous human and mouse genes. As the mRNA and EST libraries of human and mouse are not complete and thus cannot be compared directly, we instead analyzed whether known cassette exons or alternative splicing sites from one genome are conserved in the other genome. We demonstrate that about half of the analyzed genes have species-specific isoforms, and about a quarter of elementary alternatives are not conserved between the human and mouse genomes. The detailed results of this study are available at www.ig-msk.ru:8005/HMG_paper.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AIRE Protein
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Alternative Splicing*
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Animals
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Base Sequence
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Conserved Sequence*
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DNA-Binding Proteins / genetics
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Exons
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Expressed Sequence Tags
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Genome, Human*
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Humans
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Membrane Proteins / genetics
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Mice
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Nerve Tissue Proteins / genetics
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Proto-Oncogene Proteins / genetics
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RNA Splicing Factors
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RNA, Messenger / genetics
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RNA-Binding Proteins / genetics
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Sodium-Potassium-Exchanging ATPase / genetics
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Transcription Factors / genetics
Substances
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DNA-Binding Proteins
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MEN1 protein, human
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Membrane Proteins
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NNAT protein, human
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Nerve Tissue Proteins
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Nnat protein, mouse
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Proto-Oncogene Proteins
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RAD51D protein, human
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RNA Splicing Factors
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RNA, Messenger
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RNA-Binding Proteins
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Rad51d protein, mouse
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SF1 protein, human
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Sf1 protein, mouse
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Sf1 protein, rat
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Transcription Factors
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FXYD2 protein, human
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Fxyd2 protein, mouse
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Sodium-Potassium-Exchanging ATPase