Objective: To increase the number of the tumor draining lymph nodes lymphocytes (TDLNLs) in tumor site and study its anti-tumor effect.
Methods: Interleukin 2-activated TDLNLs were administrated by local injection in 10 nude mice bearing human laryngeal cancer at 10(7) cells/mouse, once a week for two weeks. As a control, 10 nude mice bearing human laryngeal cancer were injected with D-Hanks buffer solution.
Results: The volume and weight of TDLNLs treated tumors were significantly reduced in comparison with those of the control (P < 0.05). The observed histopathologic characteristics of treated tumors were tumor necrotic foci and increased infiltration of lymphocytes in tumor and around tumor vessels. Proliferation of spleen nodules was also observed in the treatment group. Remote metastasis was not found in this group. In contrast with the control group, tumor necrosis and proliferation of spleen nodules were not observed and infiltration of lymphocytes in the tumor was not significant. There was a lung metastasis in the control group.
Conclusion: Local injection of TDLNLs is effective against human laryngeal carcinoma xenografts in nude mice. It also could stimulate the immunoresponse of hosts and probably decrease the chance of tumor metastasis.