Correlated motions of successive amide N-H bonds in proteins

J Biomol NMR. 2003 Apr;25(4):265-80. doi: 10.1023/a:1023076212536.

Abstract

New nuclear magnetic resonance (NMR) methods are described for the measurement of cross-correlation rates of zero- and double-quantum coherences involving two nitrogen nuclei belonging to successive amino acids in proteins and peptides. Rates due to the concerted fluctuations of two NH(N) dipole-dipole interactions and to the correlated modulations of two nitrogen chemical shift anisotropies have been obtained in a sample of doubly labeled Ubiquitin. Ambiguities in the determination of dihedral angles can be lifted by comparison of different rates. By defining a heuristic order parameter, experimental rates can be compared with those expected for a rigid molecule. The cross-correlation order parameter that can be derived from a model-free approach can be separated into structural and dynamic contributions.

MeSH terms

  • Amides / chemistry*
  • Isotope Labeling
  • Magnetics
  • Models, Theoretical
  • Motion
  • Nitrogen Isotopes
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Proteins / chemistry*
  • Ubiquitin / chemistry

Substances

  • Amides
  • Nitrogen Isotopes
  • Proteins
  • Ubiquitin