Preliminary study of laser welding for aortic dissection in a porcine model using a diode laser with indocyanine green

Lasers Surg Med. 2003;32(5):341-5. doi: 10.1002/lsm.10177.

Abstract

Background and objectives: The objective of this study was to determine whether a dissected aorta could be welded by a diode laser with a solder using an in vitro porcine aortic dissection model.

Study design/materials and methods: Porcine aortic strips were dissected into two flaps and the dissected faces were immersed in a solution of indocyanine green. The two flaps were pressed at 0.2 kg/cm2 with contact between the two immersed faces. The pressed flaps were irradiated with a diode laser (810 nm) at intensities of 170-425 W/cm2 for 8 seconds. The welded flaps were studied by light microscopy and the adhesive strengths were measured.

Results: The irradiated flaps were successfully welded. The breaking stress, the maximum stress recorded in a stress-strain curve, increased with increase in irradiation intensity up to 396 W/cm2 (2.7 x 10(2) mmHg) and decreased when the intensity reached 425 W/cm2. In the specimen irradiated at 396 W/cm2, the welded faces showed continuous fusion of elastin layers, while some voids were seen between the welded faces in the specimen irradiated at 425 W/cm2.

Conclusions: The dissected porcine aortas were successfully welded using a laser with solder. The results suggest that the welded aorta can bear physiological blood pressure.

MeSH terms

  • Animals
  • Aortic Aneurysm / pathology
  • Aortic Aneurysm / surgery*
  • Aortic Dissection / pathology
  • Aortic Dissection / surgery*
  • Coloring Agents / therapeutic use*
  • In Vitro Techniques
  • Indocyanine Green / therapeutic use*
  • Laser Therapy / instrumentation
  • Laser Therapy / methods*
  • Models, Animal
  • Swine
  • Tensile Strength
  • Tissue Adhesives / therapeutic use*

Substances

  • Coloring Agents
  • Tissue Adhesives
  • Indocyanine Green