Abstract
We use a modified form of ChIP to analyze the recruitment of seven sets of proteins to the yeast GAL genes upon induction. We resolve three stages of recruitment: first SAGA, then Mediator, and finally Pol II along with four other proteins (including TBP) bind the promoter. In a strain lacking SAGA, Mediator is recruited with a time course indistinguishable from that observed in wild-type cells. Our results are consistent with the notion that a single species of activator, Gal4, separately contacts, and thereby directly recruits, SAGA and Mediator.
MeSH terms
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Binding Sites / genetics
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Cells, Cultured
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DNA-Binding Proteins
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Gene Expression Regulation / genetics
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Gene Expression Regulation, Fungal / genetics*
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Genes, Regulator / genetics*
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Macromolecular Substances
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Mediator Complex
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Promoter Regions, Genetic / genetics*
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RNA Polymerase II / genetics
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Reaction Time / genetics
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Saccharomyces cerevisiae Proteins / genetics*
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Trans-Activators / genetics
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Transcription Factors / genetics*
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Transcriptional Activation / genetics*
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Yeasts / genetics*
Substances
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DNA-Binding Proteins
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GAL11 protein, S cerevisiae
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GAL4 protein, S cerevisiae
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Macromolecular Substances
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Mediator Complex
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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Transcription Factors
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RNA Polymerase II