Effects of Daflon on oxidative stress induced by hindlimb ischemia/reperfusion

Pharmacol Res. 2003 Jul;48(1):11-5.

Abstract

The objective of this study was to investigate the effects of Daflon 500 mg on tissue damage in kidney after ischemia/reperfusion hindlimb, by assessing blood biochemical assay and histopathological analysis. Rats were given Daflon 80 mg x kg(-1) x day(-1) for 10 days. On 11th day of treatment, 4h ischemia followed by 4 h reperfusion period was performed on right hind limb of the rats. Control groups were given only arabic gum and were subjected to same ischemia/reperfusion period. At the end of reperfusion period, erythrocyte superoxide dismutase, Na(+)-K(+) ATPase and reduced glutathione levels were increased in the rats erythrocytes in Daflon group (P<0.01, for all). On the other hand, serum myeloperoxidase and malondialdehyde levels were significantly lower in the Daflon-received rats (P<0.01, for all). Histopathological studies demonstrated that, there was a prominent tubulointerstitial injury with loss of brush border and this degeneration was accompanied by segmental glomerular degeneration also for both control and Daflon group. Daflon-received group animals displayed significantly less periglomerular and perivascular leukocytic infiltration (P=0.015). These overall results suggest that Daflon contributes renal protection from hind limb ischemia/reperfusion injury in some degree, by decreasing systemic oxidative stress.

MeSH terms

  • Animals
  • Diosmin / pharmacology*
  • Diosmin / therapeutic use
  • Glutathione / metabolism
  • Hindlimb
  • Kidney / drug effects*
  • Kidney / metabolism
  • Kidney / pathology
  • Male
  • Malondialdehyde / metabolism
  • Nephritis, Interstitial / chemically induced
  • Nephritis, Interstitial / physiopathology
  • Oxidative Stress / drug effects*
  • Oxidative Stress / physiology
  • Peroxidase / metabolism
  • Protective Agents / pharmacology*
  • Protective Agents / therapeutic use
  • Rats
  • Rats, Wistar
  • Reperfusion Injury / drug therapy*
  • Reperfusion Injury / metabolism
  • Sodium-Potassium-Exchanging ATPase / metabolism
  • Superoxide Dismutase / metabolism

Substances

  • Protective Agents
  • Malondialdehyde
  • Diosmin
  • Peroxidase
  • Superoxide Dismutase
  • Sodium-Potassium-Exchanging ATPase
  • Glutathione