Transposition in Lactobacillus delbrueckii subsp. bulgaricus: identification of two thermosensitive replicons and two functional insertion sequences

Microbiology (Reading). 2003 Jun;149(Pt 6):1503-1511. doi: 10.1099/mic.0.25827-0.

Abstract

In this report, it is shown that the rolling circle replicon pG(+)host and the theta replicon pIP501 are thermosensitive in Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Using a pIP501 derivative as a delivery vector for six insertion sequences originating from lactic acid bacteria, it is shown that IS1223 and IS1201 transpose in L. bulgaricus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Transposable Elements / genetics*
  • DNA, Bacterial / genetics
  • Lactobacillus / genetics*
  • Molecular Sequence Data
  • Mutagenesis, Insertional / methods
  • Plasmids / genetics
  • Replicon*
  • Temperature

Substances

  • DNA Transposable Elements
  • DNA, Bacterial

Associated data

  • GENBANK/AF449484