Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer

Johanna Jefferies; Stuart C Clarke; Mathew A Diggle; Andrew Smith; Chris Dowson; Tim Mitchell

doi:10.1385/MB:24:3:303

Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer

Mol Biotechnol. 2003 Jul;24(3):303-8. doi: 10.1385/MB:24:3:303.

Authors

Johanna Jefferies¹, Stuart C Clarke, Mathew A Diggle, Andrew Smith, Chris Dowson, Tim Mitchell

Affiliation

¹ Scottish Meningococcus and Pneumococcus Reference Laboratory, North Glasgow University Hospital NHS Trust, Department of Microbiology, Stobhill Hospital, Balornock Rd., Glasgow, G21 3UW, UK.

PMID: 12777696
DOI: 10.1385/MB:24:3:303

Abstract

Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Automation
Bacterial Typing Techniques / methods*
Base Sequence
DNA, Bacterial / chemistry
DNA, Bacterial / genetics*
DNA, Bacterial / isolation & purification
Phenotype
Polymerase Chain Reaction / methods
Streptococcus pneumoniae / genetics*

Substances

DNA, Bacterial