In plants, phosphate deprivation is normally known to decrease the phospholipid content consistent with a mobilization of the phosphate reserve, and conversely to increase non-phosphorous membrane lipids such as digalactosyldiacylglycerol. We report here that unexpectedly, at an early stage of phosphate starvation, phosphatidylcholine (PC) increases transiently. We also show that a significant pool of diacylglycerol (DAG) with the same fatty acid composition as that of PC is present and moreover increases in response to phosphate deprivation. The evolution of the molecular profile of the newly synthesized galactolipids is compatible with a utilization of DAG accumulating from PC hydrolysis, achieved after selection of their acyl molecular species by the galactolipid synthesizing enzymes.