Preservation of yeast cell morphology for scanning electron microscopy using 3.28-micro m IR laser irradiation

Jan Sunner; Recep Avci; Lee Richards; Gary Groenewold; Jani Ingram; Megan Arthun

doi:10.1016/s0167-7012(03)00041-1

Preservation of yeast cell morphology for scanning electron microscopy using 3.28-micro m IR laser irradiation

J Microbiol Methods. 2003 Aug;54(2):285-7. doi: 10.1016/s0167-7012(03)00041-1.

Authors

Jan Sunner¹, Recep Avci, Lee Richards, Gary Groenewold, Jani Ingram, Megan Arthun

Affiliation

¹ Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA. [email protected]

PMID: 12782385
DOI: 10.1016/s0167-7012(03)00041-1

Abstract

As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-micro m IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Gold / chemistry
Lasers*
Microscopy, Electron, Scanning* / methods
Preservation, Biological*
Saccharomyces cerevisiae / cytology
Saccharomyces cerevisiae / radiation effects
Saccharomyces cerevisiae / ultrastructure*

Substances

Gold