Utility of an in-house mycobacteriophage-based assay for rapid detection of rifampin resistance in Mycobacterium tuberculosis clinical isolates

J Clin Microbiol. 2003 Jun;41(6):2647-9. doi: 10.1128/JCM.41.6.2647-2649.2003.

Abstract

A rapid in-house mycobacteriophage-based assay to identify multidrug resistance by detecting the rifampin susceptibility of Mycobacterium tuberculosis in a microtiter plate format was evaluated. The sensitivity, specificity, and overall accuracy of the assay were 100%. This test is rapid to perform and suitable for widespread implementation.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibiotics, Antitubercular / pharmacology*
  • Drug Resistance, Multiple, Bacterial*
  • Humans
  • Microbial Sensitivity Tests / methods
  • Mycobacteriophages / physiology*
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / virology*
  • Rifampin / pharmacology*
  • Time Factors
  • Tuberculosis / microbiology

Substances

  • Antibiotics, Antitubercular
  • Rifampin