Comparison of microscan broth microdilution, synergy quad plate agar dilution, and disk diffusion screening methods for detection of high-level aminoglycoside resistance in enterococcus species

J Clin Microbiol. 2003 Jun;41(6):2703-5. doi: 10.1128/JCM.41.6.2703-2705.2003.

Abstract

We compared the dried MicroScan microdilution panel, Synergy Quad plate agar dilution, and high-potency disk diffusion screening methods for the detection of high-level aminoglycoside resistance in 815 enterococcal bloodstream isolates. Agreement between the three methods was 99% when testing for high-level gentamicin resistance and 96% when testing for high-level streptomycin resistance.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacteremia / microbiology
  • Blood / microbiology
  • Drug Resistance, Bacterial*
  • Enterococcus / drug effects*
  • Gentamicins / pharmacology*
  • Gram-Positive Bacterial Infections / microbiology
  • Humans
  • Microbial Sensitivity Tests / methods
  • Streptomycin / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Gentamicins
  • Streptomycin