Abstract
The ligand Netrin-1 and its receptors DCC and UNC5H2 are critical for the regulation of neuronal migration in nervous system development. Here we demonstrate expression of these molecules in lung development. The mRNA expression profiles of Netrin-1, DCC and UNC5H2 are developmentally regulated during embryonic mouse lung formation. Netrin-1 shows a bimodal expression pattern with elevated mRNA levels early followed by a second peak in late gestation. Peak expression of DCC occurs early in development whereas expression of UNC5H2 peaks late in development. We also demonstrate localization of Netrin-1, DCC and UNC5H2 during the stages of lung development. We present evidence that these proteins are modulated spatially in the mesenchyme and epithelium during lung organogenesis.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Adhesion Molecules / biosynthesis
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Cell Adhesion Molecules / genetics*
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DCC Receptor
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Female
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Immunohistochemistry
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Lung / embryology*
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Lung / metabolism
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Mice
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Nerve Growth Factors / biosynthesis
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Nerve Growth Factors / genetics*
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Netrin Receptors
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Netrin-1
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Pulmonary Surfactant-Associated Protein B / metabolism
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Receptors, Cell Surface / biosynthesis
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Receptors, Cell Surface / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Tumor Suppressor Proteins / biosynthesis
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Tumor Suppressor Proteins / genetics*
Substances
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Cell Adhesion Molecules
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DCC Receptor
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Dcc protein, mouse
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Nerve Growth Factors
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Netrin Receptors
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Ntn1 protein, mouse
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Pulmonary Surfactant-Associated Protein B
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Receptors, Cell Surface
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Tumor Suppressor Proteins
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Unc5h2 protein, mouse
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Netrin-1